Transcriptome analysis shows activation of circulating CD8+ T cells in patients with severe asthma.

TitleTranscriptome analysis shows activation of circulating CD8+ T cells in patients with severe asthma.
Publication TypeJournal Article
Year of Publication2012
AuthorsTsitsiou E, Williams AE, Moschos SA, Patel K, Rossios C, Jiang X, Adams O-D, Macedo P, Booton R, Gibeon D, Chung K F, Lindsay MA
JournalJ Allergy Clin Immunol
Volume129
Issue1
Pagination95-103
Date Published2012 Jan
ISSN1097-6825
KeywordsAdult, Asthma, CD4-Positive T-Lymphocytes, CD8-Positive T-Lymphocytes, Female, Gene Expression Profiling, Gene Expression Regulation, Humans, Lymphocyte Activation, Male, MicroRNAs, Middle Aged, RNA, Messenger, RNA, Untranslated, Signal Transduction, Young Adult
Abstract

BACKGROUND: Although previous studies have implicated tissue CD4(+) T cells in the development and maintenance of the inflammatory response in asthmatic patients, little is known about the role of CD8(+) T cells. There is now accumulating evidence that microRNAs and other noncoding RNAs are important regulators of T-cell function.

OBJECTIVES: We sought to use transcriptomics to determine the activation state of circulating CD4(+) and CD8(+) T cells in patients with nonsevere and severe asthma.

METHODS: mRNA and noncoding RNA expression in circulating T cells was measured by means of microarray, quantitative real-time PCR, or both.

RESULTS: Comparison of mRNA expression showed widespread changes in the circulating CD8(+) but not CD4(+) T cells from patients with severe asthma. No changes were observed in the CD4(+) and CD8(+) T cells in patients with nonsevere asthma versus those in healthy control subjects. Bioinformatics analysis showed that the changes in CD8(+) T-cell mRNA expression were associated with multiple pathways involved in T-cell activation. As with mRNAs, we also observed widespread changes in expression of noncoding RNA species, including natural antisense, pseudogenes, intronic long noncoding RNAs (lncRNAs), and intergenic lncRNAs in CD8(+) T cells from patients with severe asthma. Measurement of the microRNA expression profile showed selective downregulation of miR-28-5p in CD8(+) T cells and reduction of miR-146a and miR-146b in both CD4(+) and CD8(+) T cells.

CONCLUSIONS: Severe asthma is associated with the activation of circulating CD8(+) T cells but not CD4(+) T cells. This response is correlated with the downregulation of miR-146a/b and miR-28-5p, as well as changes in the expression of multiple species of lncRNA that might regulate CD8(+) T-cell function.

DOI10.1016/j.jaci.2011.08.011
Alternate JournalJ. Allergy Clin. Immunol.
PubMed ID21917308