TGF-β regulates Nox4, MnSOD and catalase expression, and IL-6 release in airway smooth muscle cells.

TitleTGF-β regulates Nox4, MnSOD and catalase expression, and IL-6 release in airway smooth muscle cells.
Publication TypeJournal Article
Year of Publication2011
AuthorsMichaeloudes C, Sukkar MB, Khorasani NM, Bhavsar PK, Chung K F
JournalAm J Physiol Lung Cell Mol Physiol
Volume300
Issue2
PaginationL295-304
Date Published2011 Feb
ISSN1522-1504
KeywordsBase Sequence, Catalase, Cells, Cultured, DNA Primers, Gene Expression, Humans, Interleukin-6, Models, Biological, Myocytes, Smooth Muscle, NADPH Oxidase, Phosphatidylinositol 3-Kinases, Reactive Oxygen Species, Recombinant Proteins, Respiratory Muscles, RNA, Messenger, RNA, Small Interfering, Signal Transduction, Smad Proteins, Superoxide Dismutase, Transforming Growth Factor beta1
Abstract

Reactive oxygen species (ROS) are generated as a result of normal cellular metabolism, mainly through the mitochondria and peroxisomes, but their release is enhanced by the activation of oxidant enzymes such as NADPH oxidases or downregulation of endogenous antioxidant enzymes such as manganese-superoxide dismutase (MnSOD) and catalase. Transforming growth factor-β (TGF-β), found to be overexpressed in airway smooth muscle (ASM) from asthmatic and chronic obstructive pulmonary disease patients, may be a pivotal regulator of abnormal ASM cell (ASMC) function in these diseases. An important effect of TGF-β on ASMC inflammatory responses is the induction of IL-6 release. TGF-β also triggers intracellular ROS release in ASMCs by upregulation of NADPH oxidase 4 (Nox4). However, the effect of TGF-β on the expression of key antioxidant enzymes and subsequently on oxidant/antioxidant balance is unknown. Moreover, the role of redox-dependent pathways in the mediation of the proinflammatory effects of TGF-β in ASMCs is unclear. In this study, we show that TGF-β induced the expression of Nox4 while at the same time inhibiting the expression of MnSOD and catalase. This change in oxidant/antioxidant enzymes was accompanied by elevated ROS levels and IL-6 release. Further studies revealed a role for Smad3 and phosphatidyl-inositol kinase-mediated pathways in the induction of oxidant/antioxidant imbalance and IL-6 release. The changes in oxidant/antioxidant enzymes and IL-6 release were reversed by the antioxidants N-acetyl-cysteine (NAC) and ebselen through inhibition of Smad3 phosphorylation, indicating redox-dependent activation of Smad3 by TGF-β. Moreover, these findings suggest a potential role for NAC in preventing TGF-β-mediated pro-oxidant and proinflammatory responses in ASMCs. Knockdown of Nox4 using small interfering RNA partially prevented the inhibition of MnSOD but had no effect on catalase and IL-6 expression. These findings provide novel insights into redox regulation of ASM function by TGF-β.

DOI10.1152/ajplung.00134.2010
Alternate JournalAm. J. Physiol. Lung Cell Mol. Physiol.
PubMed ID21131394