A simple data-adaptive probabilistic variant calling model.

TitleA simple data-adaptive probabilistic variant calling model.
Publication TypeJournal Article
Year of Publication2015
AuthorsHoffmann S, Stadler PF, Strimmer K
JournalAlgorithms Mol Biol
Volume10
Pagination10
Date Published03/2015
ISSN1748-7188
Abstract

BACKGROUND: Several sources of noise obfuscate the identification of single nucleotide variation (SNV) in next generation sequencing data. For instance, errors may be introduced during library construction and sequencing steps. In addition, the reference genome and the algorithms used for the alignment of the reads are further critical factors determining the efficacy of variant calling methods. It is crucial to account for these factors in individual sequencing experiments.

RESULTS: We introduce a simple data-adaptive model for variant calling. This model automatically adjusts to specific factors such as alignment errors. To achieve this, several characteristics are sampled from sites with low mismatch rates, and these are used to estimate empirical log-likelihoods. The likelihoods are then combined to a score that typically gives rise to a mixture distribution. From this we determine a decision threshold to separate potentially variant sites from the noisy background.

CONCLUSIONS: In simulations we show that our simple model is competitive with frequently used much more complex SNV calling algorithms in terms of sensitivity and specificity. It performs specifically well in cases with low allele frequencies. The application to next-generation sequencing data reveals stark differences of the score distributions indicating a strong influence of data specific sources of noise. The proposed model is specifically designed to adjust to these differences.

DOI10.1186/s13015-015-0037-5
Alternate JournalAlgorithms Mol Biol
PubMed ID25788974
PubMed Central IDPMC4363181