A metabolic system-wide characterisation of the pig: a model for human physiology.

TitleA metabolic system-wide characterisation of the pig: a model for human physiology.
Publication TypeJournal Article
Year of Publication2011
AuthorsMerrifield CA, Lewis M, Claus SP, Beckonert OP, Dumas M-E, Duncker S, Kochhar S, Rezzi S, Lindon JC, Bailey M, Holmes E, Nicholson JK
JournalMol Biosyst
Volume7
Issue9
Pagination2577-88
Date Published2011 Sep
ISSN1742-2051
KeywordsAnimals, Humans, Magnetic Resonance Spectroscopy, Male, Metabolome, Models, Animal, Swine
Abstract

The pig is a single-stomached omnivorous mammal and is an important model of human disease and nutrition. As such, it is necessary to establish a metabolic framework from which pathology-based variation can be compared. Here, a combination of one and two-dimensional (1)H and (13)C nuclear magnetic resonance spectroscopy (NMR) and high-resolution magic angle spinning (HR-MAS) NMR was used to provide a systems overview of porcine metabolism via characterisation of the urine, serum, liver and kidney metabolomes. The metabolites observed in each of these biological compartments were found to be qualitatively comparable to the metabolic signature of the same biological matrices in humans and rodents. The data were modelled using a combination of principal components analysis and Venn diagram mapping. Urine represented the most metabolically distinct biological compartment studied, with a relatively greater number of NMR detectable metabolites present, many of which are implicated in gut-microbial co-metabolic processes. The major inter-species differences observed were in the phase II conjugation of extra-genomic metabolites; the pig was observed to conjugate p-cresol, a gut microbial metabolite of tyrosine, with glucuronide rather than sulfate as seen in man. These observations are important to note when considering the translatability of experimental data derived from porcine models.

DOI10.1039/c1mb05023k
Alternate JournalMol Biosyst
PubMed ID21761043