[Immunohistochemical research on human breast tumors using monoclonal antibodies to intermediate filament proteins. Cancer of the breast].

Title[Immunohistochemical research on human breast tumors using monoclonal antibodies to intermediate filament proteins. Cancer of the breast].
Publication TypeJournal Article
Year of Publication1986
AuthorsGel'shteĭn VI, Chipysheva TA, Ermilova VD, Litvinova LV, Bannikov GA
JournalArkh Patol
Volume48
Issue8
Pagination14-22
Date Published1986
ISSN0004-1955
KeywordsAntibodies, Monoclonal, Breast, Breast Neoplasms, Epithelium, Female, Fluorescent Antibody Technique, Histocytochemistry, Humans, Intermediate Filament Proteins
Abstract

Immunomorphologic study of 29 breast cancer cases using monoclonal antibodies to proteins of intermediate filaments shown to differentiate the lining epithelium from myoepithelium in the non-proliferating epithelial structures of the mamma, has shown the cells in the majority of tumours (according to the International WHO Classification defined as infiltrating ductal, lobular, and tubular cancer forms) to contain prekeratin (PK) C12, specific for normal lining epithelium, but not for the myoepithelium. In cases of cancer with chondroid metaplasia (a malignant variant of the so-called "mixed tumour") the cells contained PK E3, vimentin and structural myosin, normally specific for myoepithelium. The cell heterogenicity in PK C12 content or its absence noted in the infiltrating cancers with predominance of a solid component can indicate a high degree of tumour anaplasia. It is concluded that usage of monoclonal antibodies to PK C12, invariably found in the cells of fibrotic invasion foci, can be a useful indicator for early diagnosis of infiltrative tumour growth.

Alternate JournalArkh. Patol.
PubMed ID3535748