Biochemical diversity of betaines in earthworms.

TitleBiochemical diversity of betaines in earthworms.
Publication TypeJournal Article
Year of Publication2013
AuthorsLiebeke M, Bundy JG
JournalBiochem Biophys Res Commun
Volume430
Issue4
Pagination1306-11
Date Published2013 Jan 25
ISSN1090-2104
KeywordsAnimals, Betaine, Magnetic Resonance Spectroscopy, Oligochaeta
Abstract

The ability to accumulate osmoprotectant compounds, such as betaines, is an important evolutionary feature in many organisms. This is particularly the case for organisms that live in variable environments, which may have fluctuations in moisture and salinity levels. There is, surprisingly, very little known about betaines in soil invertebrates in general, and there is almost no information about earthworms - a group that are important 'ecosystem engineers' and key indicators of soil health. Here, we describe a fast and reliable (1)H-(13)C heteronuclear single quantum coherence (HSQC) 2D NMR approach for the metabolic profiling of a series of betaines and related metabolites in tissue extracts, and list (1)H and (13)C chemical shifts for the trimethylammonium signal for 23 such compounds. The analysis of ten different species from three different families (Lumbricidae, Megascolecidae and Glossoscolecidae) showed an unexpected diversity of betaines present in earthworms. In total ten betaines were identified, including hydroxyproline-betaine, proline-betaine, taurine-betaine, GABA-betaine and histidine-betaine, and a further eleven as-yet unassigned putative betaine metabolites detected. The findings clearly indicate a hitherto-unappreciated important role for betaine metabolism in earthworms.

DOI10.1016/j.bbrc.2012.12.049
Alternate JournalBiochem. Biophys. Res. Commun.
PubMed ID23261439